T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′ phosphate and 3′ hydroxyl termini in duplex DNA or RNA.
ZERO BIAS – scores, article reviews, protocol conditions and more. ( A ) The ligation products joined by using T4 DNA ligase from Fermentas.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5′-phosphate and 3′-hydroxyl termini in duplex DNA or RNA. The enzyme repairs
The T4 DNA Ligase requires ATP as a cofactor. Self-circularization of linear DNA. Binding of T4 DNA Ligase to DNA may result in a band shift in agarose gelsThe kit contains T4 DNA ligase and a specially-formulated 5X rapid ligation buffer optimized for fast and efficient DNA ligation. Fast ligation efficiency is
T4 DNA ligase is an Mg2+-dependent and ATP-dependent enzyme that seals DNA nicks in three steps: it covalently binds AMP, transadenylates the nick phosphate,